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. 2015 Dec 9;10(12):e0144254. doi: 10.1371/journal.pone.0144254

Fig 4. Double and stable transfected cells by W/O droplet electroporation.

Fig 4

(A), (B) Transgene plasmid DNAs of two kinds of fluorescent protein: green fluorescent protein (GFP) and Tag-RFP (red fluorescent protein), were successfully double transfected into HEK293 cells by W/O droplet electroporation for 3 minutes. Bright-field image and fluorescent GFP or RFP image of HEK293 cells (A) 5 days (71% of cells maintained GFP and 71% of cells maintained TagRFP signal) and (B) 8 days (41% of cells maintained GFP and 37% of cells maintained TagRFP signal) after W/O droplet electroporation. Scale bars, 30 μm. (C) Transgene plasmid DNAs of Venus and mCherry (red FP) were successfully double transfected into hippocampus primary neural cell lines by W/O droplet electroporation for 5 minutes. Scale bars, 30 μm. (D), (E) Bright-field image and fluorescent image of Venus and mCherry expression in human fibroblast cells (D) 2 days and (E) 10 days after W/O droplet electroporation. Scale bars, 30 μm.