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. 2015 Dec 9;10(12):e0144226. doi: 10.1371/journal.pone.0144226

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© 2015 Zheng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) Microscopic image showing the morphology of SFMSCs after transfection for 4 days. (B) Microscopic image showing the morphology of SFMSCs after transfection for 20 days. (C) Typical image of hES cell-like colony after culture with Matrigel and mTeSR1 medium culture. (D) High-magnification image of SFMSC-iPSCs. (E) Staining of SFMSC-iPSCs showing positive expression of alkaline phosphatase. (F) Floating culture of SFMSC-iPSCs at day 8. Immunofluorescent staining of SFMSC-iPSCs showing positive expression of NANOG (G), OCT-4 (H), SOX-2 (I), SSEA-4 (J), TRA-1-60 (K), and TRA-1-81 (L). Scale bars = 100 μm.