Table 1. Oligonucleotide primers used in qPCR.
Gene | Primer sequence |
---|---|
GAPDH | Forward: GACAGTCAGCCGCATCTTCT |
Reverse: TTAAAAGCAGCCCTGGTGAC | |
RUNX-2 | Forward: TCAACGATCTGAGATTTGTGGG |
Reverse: GGGGAGGATTTGTGAAGACGG | |
OCN | Forward: CCACCGAGACACCATGAGAG |
Reverse: TCAGCCAACTCGTCACAGTC | |
PPARG2 | Forward: GCAAACCCCTATTCCATGCTG |
Reverse: CACGGAGCTGATCCCAAAGT | |
LPL | Forward: CAAGAGTGAGTGAACAAC |
Reverse: AATTATGCTGAAGGACAAC | |
SOX9 | ACACACAGCTCACTCGACCTTG |
AGGGAATTCTGGTTGGTCCTCT |
Abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; RUNX-2, runt-related transcription factor 2; OCN, osteocalcin; PPARG2, peroxisome proliferator-activated receptor gamma, transcript variant 2; LPL, lipoprotein lipase; SOX9, sex determining region Y-box 9.