Skip to main content
. 2015 Dec 9;10(12):e0142041. doi: 10.1371/journal.pone.0142041

Fig 1. Effects of KBH-1 on lipid accumulation and proliferation in 3T3-L1 adipocyte.

Fig 1

3T3-L1 preadipocytes were induced to differentiate into mature adipocytes in the presence of KBH-1. (A) Lipid accumulation was measured using Oil Red O staining at a concentration of 10 μg/ml KBH-1, (B) intracellular triglyceride (TG) content was measured using the AdipoRed assay in the presence of KBH-1 (0–200 μg/ml). (C) Cell proliferation was examined using MTT assay for 24 and 72 h in the presence of KBH-1 (0–200 μg/ml). (D)Cell cycle distribution was evaluated or 24 h in the presence of 10 μg/ml of KBH-1 using flow cytometry. (E) Cell viability was examined on day 7 using cell count kit-8. Data are expressed as the mean ± SEM. Significant differences from control (0 μg/ml) are indicated by ***p < 0.001.