Fig 5. Effects of siRNA for AMPK on KBH-1-induced inhibition of adipocyte differentiation.

3T3-L1 preadipocytes were induced to differentiate into mature adipocytes in the presence of KBH-1. Final concentration of 50 nM si RNA for AMPK (si-AMPK) was incubated with 3T3-L1 preadipocyte for 72 h, and then transfection medium was removed and cells were differentiated in the same condition as normal differentiation. (A) Lipid accumulation was measured using Oil Red O staining at a concentration of 10 μg/ml KBH-1 on day 7. (B) AMPK, ACC and PPARγ phosphorylation were measured using SDS-PAGE and immunoblotting. Bar graph (right panel) is the relative density after normalization to β-actin. Data are expressed as the mean ± SEM. Significant differences from the band of no si-AMPK treatment in the presence of KBH-1 are indicated by ** p < 0.01.