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. 2015 Dec 9;10(12):e0144170. doi: 10.1371/journal.pone.0144170

Fig 1. Performance of the cBRAF V595E assay in a serial dilution of plasmid containing V595E sequence in wild type dog gDNA.

Fig 1

(A) Representative two-dimensional scatter plots of the ddPCR assay in serially-diluted DNA samples. Areas in red rectangles indicate the positive reactions for cBRAF V595E assay. The numbers shown in red rectangles indicate the number of droplets positive for the V595E mutation assay. (B) Correlation of V595E fraction calculated by the dilutions and measured by ddPCR. The V595E mutation was detected up to 0.005% of V595E fraction. Vertical error bars represents the 95% confidence intervals calculated by the Poisson distribution.