Agarose gel electrophoresis showing the analytical sensitivity of nested polymerase chain reaction (PCR). The PCR products are as follows: lane 1, DNA from one Strongyloides stercoralis filariform larva; lane 2, 10−1 dilution of DNA from one S. stercoralis filariform larva; lane 3, 10−2 dilution of DNA from one S. stercoralis filariform larva; lane 4, 10−3 dilution of DNA from one S. stercoralis filariform larva; lane 5, 10−4 dilution of DNA from one S. stercoralis filariform larva; lane 6, 10−5 dilution of DNA from one S. stercoralis filariform larva; lane 7, negative control; and lane 8, positive control (S. stercoralis). M = 100-bp DNA marker.