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Experiment design for the feasibility study of using blood meal in mosquito abdomens for blood-borne virus surveillance. Blood spiked with dengue virus type 2 (DENV-2) was used to feed Anopheles stephensi mosquitoes. Controls and mosquito blood meal specimens, kept in vials at −80°C or on FTA cards at room temperature, were subjected to storage, nucleic acid extraction, and analyses by real-time reverse-transcription polymerase chain reaction (qRT-PCR) or next-generation sequencing.
