Recovery of nucleic acids from Whatman FTA cards. Aliquots of artificial whole blood with dengue virus type 2 (DENV-2) were collected before and after feeding mosquitoes, kept in vial (empty bars) or spotted on FTA cards (gray bars), and subjected to storage at −80°C and room temperature for 4 weeks, respectively, nucleic acids extraction, and quantitation by real-time reverse-transcription polymerase chain reaction (qRT-PCR). The error bars are standard deviations of data for eight samples.