Table 1.
MiSeq sequencing of FTA dried blood spots and controls
| Sample* | Description | MiSeq reads | DENV reads | DENV (%) | DENV per million reads |
|---|---|---|---|---|---|
| CB0 | Artificial blood meal, DENV-2, before feeding | 165,220 | 93,393 | 56.526 | 565,264 |
| CF0 | CB0 on FTA | 65,458 | 9,183 | 14.029 | 140,288 |
| CB1 | Artificial blood meal, DENV-2, after feeding | 470,674 | 216,365 | 45.969 | 459,692 |
| CF1 | CB1 on FTA | 55,852 | 1,981 | 3.547 | 35,469 |
| B0 | Anopheles stephensi midgut, 0 hour after feeding | 279,856 | 15 | 0.005 | 54 |
| B4 | Midgut, 4 hours | 301,510 | 65 | 0.022 | 216 |
| B8 | Midgut, 8 hours | 277,882 | 48 | 0.017 | 173 |
| B16 | Midgut, 16 hours | 299,418 | 39 | 0.013 | 130 |
| B24 | Midgut, 24 hours | 173,214 | 2 | 0.001 | 12 |
| F0 | An. stephensi midgut blotted on FTA, 0 hour after feeding | 310,056 | 16 | 0.005 | 52 |
| F4 | Midgut on FTA, 4 hours | 134,162 | 23 | 0.017 | 171 |
| F8 | Midgut on FTA, 8 hours | 281,964 | 20 | 0.007 | 71 |
| F16 | Midgut on FTA, 16 hours | 147,968 | 97 | 0.066 | 656 |
| F24 | Midgut on FTA, 24 hours | 338,480 | 19 | 0.006 | 56 |
| NTC† | No template reaction and process control | 251,720 | 2 | 0.001 | 8 |
DENV = dengue virus; NTC = no template controls.
*
CB and B samples, aliquots stored in vials at −80°C; CF and F samples, dried blood spots on FTA cards stored at room temperature.
†
Water was used instead of nucleic acids to detect background contamination from the process in laboratory.