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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Microbiol. 2015 Jul 17;97(6):1097–1113. doi: 10.1111/mmi.13088

Figure 1. Rgg-SHP signaling under metal limitation.

Figure 1

(A) Growth and (B, C) luciferase activity of wild-type NZ131 and isogenic Pshp3-luxAB reporter strains grown in metal-deplete or replete conditions. Cells were grown to mid-log in CDM+1% glucose, washed three times with CxCDM containing 100 μM dipyridyl, then diluted into CxCDM supplemented with 50 μM CaCl2and 1000 μM MgSO4 (deplete) or 50 μM CaCl2, 1000 μM MgSO4, 20 μM FeSO4, and 30 μM MnSO4 (replete). (D) Growth and (E) luciferase activity of the wild-type NZ131 reporter strain grown in decreasing amounts of iron and manganese. CxCDM was supplemented with with 50 μM CaCl2, 1000 μM MgSO4, and equal amounts of FeSO4 and MnSO4 as indicated. Data are representative of experiments performed at least three times.