(A) Putative mannose PTS operons in S. pyogenes. Light gray genes indicate PTS components; IIC components targeted for insertional disruption are shown in dark gray. (B, D, F) Growth and (C, E, G) luciferase activity of manM
(B, C), ptsC
(D, E), and agaW
(F, G) mutants grown in different carbon sources (at 1%). For luciferase experiments, 50 nM synthetic C8 peptide was added to CDM+1% glucose as a positive control for Pshp3 induction. Data are representative of experiments performed at least three times.