Skip to main content
. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Mol Microbiol. 2015 Jul 17;97(6):1097–1113. doi: 10.1111/mmi.13088

Figure 4. Metal limitation and carbon source availability elicit distinct responses upstream of pheromone production.

Figure 4

(A) Luciferase activity of wild-type and ptsC Pshp3-luxAB reporter strains grown in metal-deplete or replete conditions. CxCDM was supplemented with 50 μM CaCl2and 1000 μM MgSO4 (deplete) or 50 μM CaCl2, 1000 μM MgSO4, 20 μM FeSO4, and 30 μM MnSO4 (replete). Data are representative of experiments performed at least three times. (B) Luciferase expression from rgg2 and rgg3 promoters under different growth conditions. Bars 1–3: Luciferase activity in 1% mannose or 50 nM synthetic SHP3-C8 after three hours of growth was normalized to expression in 1% glucose. Bars 4–5: Luciferase activity of cells grown under metal deplete conditions was normalized to promoter activity in replete conditions. The mean and SD of at least three independent samples are shown.