Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 7;211(5):1057–1075. doi: 10.1083/jcb.201404147

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Kumar et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Isotype-specific keratin expression and localization in KtyI^−/− and KtyII^−/−_K8 epidermis. (A) Immunofluorescence analysis of keratin localization in WTs and KtyI^−/− and KtyII^−/−_K8 epidermis at E18.5. Note absence of KIF in both KOs, suprabasal aggregates of Krt1 and Krt5 in KtyI^−/−, and widespread aggregates of Krt14, but not of Krt10, in KtyII^−/−_K8. Basement membrane is indicated by white dotted lines. Bars, 20 µm. (B) Western blot analysis of Krt1, Krt5, Krt6, Krt10, Krt14, and α-tubulin as loading control in skin extracts of WT and keratin-deficient embryos at E18.5. Note positive correlation with A, showing a significant increase in Krt6 in KtyI^−/− but unaltered presence of Krt14 in KtyII^−/−_K8 compared with KtyII^+/+_K8.