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. 2015 Dec 7;211(5):1057–1075. doi: 10.1083/jcb.201404147

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© 2015 Kumar et al.

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Figure 6. — Induction of Nrf2 in KtyI^−/− and KtyII^−/−_K8 prenatal epidermis. (A) Strong upregulation of selected Nrf2 target genes from transcriptome profiles of E18.5 KtyI^−/− and KtyII^−/−_K8 skin RNA. (B) Confirmation of data in A, using quantitative RT-PCR analysis. (C) Transcriptome profile and quantitative RT-PCR showing increase in Nrf2 mRNA in the skin of keratin-deficient embryos. (D) Western blot establishing a strong increase of Nrf2 protein in KtyI^−/− and KtyII^−/−_K8 at E17.5 and E18.5, but no difference in Keap1. (E) Persistent upregulation and nuclear localization of Nrf2 in the epidermis of both KO mice, based on immunohistochemistry of E16.5–E18.5 embryos. Bars: (main) 50 µm; (insets) 10 µm. *, P ≤ 0.05.