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. 2015 Dec 7;211(5):1041–1056. doi: 10.1083/jcb.201412066

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© 2015 Motley et al.

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Figure 2. — De novo–formed peroxisomes grow into elongated peroxisomes in DRP-deficient cells. (A) Galactose-controllable PEX19 strains transformed with constitutive HcRed-PTS1 were grown on raffinose (top) or galactose medium for the times indicated in B. (B) Peroxisome number per cell was determined for at least 200 cells for each time point. Blue, conditional pex19 expression in WT background. Red, conditional pex19 expression in vps1Δ/dnm1Δ background. (C) Stills of a time-lapse video (Video 1): peroxisomes formed de novo in vps1/dnm1 cells expressing HcRed-PTS1 segregate on cell division, until a single enlarged peroxisome remained. Asterisks indicate cells that move away. Unit of measure is in minutes. Arrows indicate new buds forming. Bars, 5 µm.