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. 2015 Dec 7;211(5):1041–1056. doi: 10.1083/jcb.201412066

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© 2015 Motley et al.

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Figure 3. — Quantitation of membrane structures in pex1Δ, pex1Δ/atg3Δ6, vpsΔ1/dnm1Δ, and vps1Δ/dnm1Δ/pex1Δ/atg3Δ6 cells. Mutants expressing Pex11- or Pex13-GFP from their endogenous promoters (on plasmids) were imaged by epifluorescence microscopy. Cells were kept in a log phase on glucose-containing medium for 18 h before imaging. The doubling time of WT, vps1Δ/dnm1Δ, pex1Δ/atg36Δ, and vps1Δ/dnm1Δ/pex1Δ/atg36Δ strains under this condition was 101 min, 100 min, 106 min, and 115 min, respectively. The number of Pex13-GFP puncta per cell was determined for at least 200 cells per strain. Bar, 5 µm.