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. 2015 Dec 7;211(5):1041–1056. doi: 10.1083/jcb.201412066

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© 2015 Motley et al.

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Figure 4. — Pex1 is not required for de novo formation of peroxisomal membranes. (A) Galactose-controllable PEX19 strains expressing Pex13-GFP and Pex11-mRFP from their endogenous promoters (on plasmids) were grown on raffinose (top) or galactose medium for the times indicated. The Pex11-mRFP signal was weak and was enhanced strongly to show localization. (B) WT, pex3Δ, and pex19Δ cells expressing Pex11-mRFP from its endogenous promoter on plasmid were grown to log phase and stained with MitoTracker green. (C) WT and pex19Δ cells expressing Pex13-GFP and Pex3-mCherry (Pex3-mCH) from endogenous promoters (on plasmids) were grown to log phase. Bars, 5 µm.