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. 2015 Dec 7;211(5):1041–1056. doi: 10.1083/jcb.201412066

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© 2015 Motley et al.

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Figure 6. — Colocalization of Pex11 and Pex13 in pex1 and pex6 cells. (A) pex1, pex6, pex1/pex6, and WT cells expressing Pex11-RFP and Pex13-GFP (from endogenous promoters on plasmids) were imaged after 3-h growth in log phase. (B) The distance between the center of each fluorescent Pex11-RFP and Pex13-GFP spot was calculated as described in the Materials and methods subsection Image acquisition and processing and is depicted as a box plot. n > 400 spots for each strain. The pale green line across each box indicates the median. (C) vps1/dnm1/pex1/atg36 cells expressing Pex11-RFP and Pex13-GFP (from endogenous promoters on plasmid) were imaged after 18 h in exponential growth phase. (D) WT and pex1/pex6 cells expressing plasmid-based Pex13-mCherry constitutively and Pex11-GFP from the GAL1 promoter were given a short pulse of Pex11-GFP as indicated. Bars, 5 µm.