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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2015 Oct 5;14(12):2797–2804. doi: 10.1158/1535-7163.MCT-15-0291-T

Figure 1. Chemically synthesized A10-3-DNAPK siRNA chimera.

Figure 1

A, Schematic of three parts annealed chimera. A10-3: PSMA targeting 2’-Fluorpyrimdine aptamer; S-siRNA: Non-modified sense siRNA with a 3’ extended bridge for aptamer annealing; AS-siRNA: Non-modified antisense siRNA. Both sense and antisense siRNA contain unpaired 3’-UU-dinucleotides. B, Annealed products. A10-3-DNAPK: A10-3, DNA-PK sense siRNA with bridge and DNA-PK antisense RNA; siDNAPK: DNA-PK sense siRNA with bridge and DNA-PK antisense RNA; A10-3-Con: A10-3, control sense siRNA with bridge and control antisense RNA; siCon: control sense siRNA with bridge and control antisense RNA. C, A10-3-DNAPK siRNA +/− Dicer processing in vitro. ds-siDNAPK applied for size reference. Images (B,C) represent ethidium bromide stained polyacrylamide gels.