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. Author manuscript; available in PMC: 2016 Dec 8.
Published in final edited form as: Circulation. 2015 Oct 27;132(23):2237–2247. doi: 10.1161/CIRCULATIONAHA.115.016540

Figure 7.

Figure 7

Fn-EDA potentiates canonical NFκB signaling via TLR4. Bone marrow-derived neutrophils from Fn-EDA−/−Apoe−/− and Fn-EDA−/−TLR−/−Apoe−/− mice were stimulated with 20 ng phorbol myristate acetate in the presence or absence of cFn (10µg/well) for 24 hours. Left panel shows representative immunoblots of phospho- NFκB p65 and total NFκB p65. β actin was used as a loading control. The right panel represents quantification of intensity of phospho-NFκB p65 to total NFκB p65 in the presence or absence of cFn. Data are presented as mean ± SEM. N = 5/group. B &C. Quantification of TNFα and IL-1β by ELISA. Data are presented as mean ± SEM. N = 5/group.