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. Author manuscript; available in PMC: 2016 Oct 24.
Published in final edited form as: Curr Opin Genet Dev. 2015 Oct 24;34:82–87. doi: 10.1016/j.gde.2015.09.002

Figure 1.

Figure 1

Global DNA methylation remodeling occurs in vivo during pre-implantation embryo development and in primordial germ cells (PGCs). In PGCs demethylation occurs in two stages as represented by two arrows. Global DNA methylation remodeling in vitro occurs during culture-induced reversion of PGCs to embryonic germ cells (EGCs) in medium containing 2i+LIF (called “2i” in the text), reversion of mouse ESCs cultured in a medium containing serum + LIF (called “serum” in the text) to 2i, and reversion of primed hESCs cultured in FGF2/Knockout Serum replacer (KSR) to the naïve state in a medium called titrated 2i + LIF + Gö6983.