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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2015 Oct 6;14(12):2789–2796. doi: 10.1158/1535-7163.MCT-15-0532

Figure 3.

Figure 3

Stimulation of human PBMC with LMB-T20 results in a significantly diminished T cell epitopes. PBMC from 10 donors were plated in pre-coated blocked ELISpot plates and stimulated with 50 μg/ml of LMB-T20, SS1P or KLH or nothing diluted in media. After 6 days cells were treated with a second boost of 50 μg/ml of the respective proteins. T cell activation was evaluated using IFNγ and IL-2 ELISpot. A. IL-2 response. B. IFNγ response. Lines represent medians. **** p<0.0001 ***p<0.001 and **p<0.01 in Freidman test with Dunn’s multiple comparisons. C. Representative ELISpot wells after spot detection. All donors and stimulations were run in four replicas and the assay was repeated for 10 of the 12 samples. The other two samples were not repeated because the PBMC samples were not abundant enough for two experiments. One sample was invalidated because the response did not repeat in the second IL-2 assay.