Figure 6.

Effect of deimmunized RITs on mouse xenograft tumor model. Arrows indicate the days that treatment was administered. (A) Female athymic nude mice were inoculated with 10⁶ A431/H9 cells at time 0. Intravenous treatment with LMB-T20 (5mg/kg) or vehicle began on day 5 and continued every day for a total of six doses. Average of mean tumor volume for n = 8 animals treated with LMB-T20 or vehicle. On day 38 one of the mice in LMB-T20 group was euthanized due to tumor burden and thus averages after this time point cannot be shown. A significant decrease in tumor size was observed on day 9 (p<0.01 in Mann Whitney test) and every measurement after that time point until day 38. (B) Female athymic nude mice were inoculated with 4×10⁶ KLM1 cells at time 0. Intravenous treatment with LMB-T20 (5mg/kg) or vehicle began on day 7 and continued every day for a total of seven doses. Each data point represents average of mean tumor volume for n = 5 animals treated with LMB-T20 or vehicle. Significant differences between tumor size was observed between the two treatment groups starting on day 9 (p<0.05 in Mann Whitney test). On day 29 the experiment was terminated when all mice in the treated group had tumors >500mm³. (C) Female SCID mice were injected with cells obtained from ascitic fluids of patient NCI-M-16. On day 53, when tumor reached 100mm³, six doses of 5mg/kg LMB-T20 were injected intravenously every day. A significant difference in tumor size was observed beginning on day 55 (P<0.03) in Mann Whitney test. (D) Ten SCID mice were injected with cells obtained from ascites of patient NCI-M-21. On day 88, six doses of 5mg/kg LMB-T20 were injected intravenously every other day. A significant difference in tumor size was observed beginning on day 90 (P<0.007) in Mann Whitney test. Error bars indicate SEM.