FIGURE 1.

The solubilized grana cores (SG), when resolved by Blue native polyacrylamide gel electrophoresis (BN-PAGE; A) separated into a pattern of bands equivalent to specific thylakoid complexes. The bands were attributed to a given complex on the basis of their mass spectrometry (MS) analysis (see Table 1). The PSII pools purified by NiNTA affinity chromatography are resolved by BN-PAGE (B). The lane SG is the PSII pool purified from the solubilized grana fraction, whereas lane SL represents the pool of PSII purified from solubilized lamellae. In lanes M the molecular marker (M) was loaded. C₂S₂M₂, C₂S₂, C₂ and C are the PSII-LHCII megacomplexes, PSII-LHCII supercomplexes, PSII dimers and PSII monomers, respectively. ^∗The BN-PAGE used must be considered reliable in the mass range between 66 and 480 kDa according to the molecular marker (M). Above this range, the heaviest bands appear significantly under estimated in weight.