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. 2015 Dec 10;6:1100. doi: 10.3389/fpls.2015.01100

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Copyright © 2015 Haniewicz, Floris, Farci, Kirkpatrick, Loi, Büchel, Bochtler and Piano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Size exclusion chromatography of the PSII pool isolated from SG thylakoids by affinity chromatography (solid lines). All the measurements were recorded at three different wavelengths: 280 nm (proteins), 664 nm (chlorophyll a), 647 nm (chlorophyll b). In the inset are shown the elution fractions analyzed by BN-PAGE, confirming the partial separation of several oligomeric states of PSII. M is the molecular marker. The mostly monomeric PSII pool isolated from SL thylakoids by affinity chromatography (dotted lines) was used as a mass reference. In the chromatograms and in the inset SG:C₂S₂M₂, SG:C₂S₂, SG:C₂, and SG:C are the PSII-LHCII megacomplexes, PSII-LHCII supercomplexes, PSII dimers, and PSII monomers of grana origin (SG), respectively; SL:C are PSII monomer from lamellae (SL).