Table 3.
Mass spectrometry analysis performed on the bands of the SEC fractions resolved by BN-PAGE.
| Name | Accession Number | Mass (kDa) | Qualitative (unweighted subunit presence) |
Quantitative∗ (weighted subunit presence) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| l | C2 | C2S2 | C2S2M2 | l | C2 | C2S2 | C2S2M2 | |||||
| PSII-LHCII (super/megacomplexes) | PSII monomers and dimers | PSB_A (D1) | PSBA_TOBAC | 39 | + | + | + | + | +++ | +++ | +++ | +++ |
| PSB_B (CP47) | PSBB_TOBAC | 56 | + | + | + | + | +++ | +++ | +++ | +++ | ||
| PCB_C (CP43) | PSBC_TOBAC | 52 | + | + | + | + | +++ | +++ | +++ | +++ | ||
| PSB_D (D2) | PSBD_TOBAC | 40 | + | + | + | + | +++ | +++ | +++ | +++ | ||
| PSB_E (cytb559) | PSBE_TOBAC | 9 | + | + | + | + | +++ | +++ | +++ | + | ||
| PSB_H | PSBH_TOBAC | 8 | + | + | + | + | +++ | +++ | +++ | +++ | ||
| PSB_L | PBL_TOBAC | 4 | – | + | + | – | – | +++ | +++ | – | ||
| PSB_O (33kDa) | Q84QE8 | 35 | + | + | + | + | – | ++ | +++ | + | ||
| PSB_O (33kDa) | PSBO_TOBAC | 35 | – | + | + | + | – | ++ | +++ | + | ||
| PSB_R | PSBR_TOBAC | 14 | – | + | + | + | – | + | + | – | ||
| CAB and LhcII proteins | Lhcb1 (CB24) | CB24_TOBAC | 28 | + | + | + | + | – | – | +++ | +++ | |
| Lhcb1 (CB27) | CB27_TOBAC | 28 | – | + | + | + | – | – | +++ | +++ | ||
| Lhcb1 (CB22) | CB22_TOBAC | 28 | – | – | + | + | – | – | +++ | ++ | ||
| Lhcb1 (CB25) | CB25_TOBAC | 28 | – | – | + | – | – | – | +++ | – | ||
| Lhcb2 (CB23) | CB23_TOBAC (+1) | 29 | – | + | + | + | – | – | +++ | ++ | ||
| Lhcb3 | A0A076L1Y1_TOBAC (+1) | 29 | – | – | + | + | – | – | – | – | ||
| Lhcb4 (CP29) | Q0PWS7_TOBAC | 31 | + | + | + | + | – | – | +++ | +++ | ||
| Lhcb5 (CP26) | Q0PWS5_TOBAC | 30 | – | + | + | + | – | – | +++ | +++ | ||
| Lhcb6 (CP24) | Q0PWS6_TOBAC | 27 | – | + | + | + | – | – | + | + | ||
| Plastidial ATP synthase | ATP_A (α-subunit) | ATPA_TOBAC | 55 | – | + | – | – | – | – | – | – | |
| ATP_B (β-subunit) | ATPB_TOBAC (+1) | 54 | – | + | – | – | – | – | – | – | ||
| – | 37kDa inner membrane polypeptide | Q40501_TOBAC | 38 | – | + | + | + | – | – | – | – | |
| Vacuolar H+-ATPase | subunit B | Q9M5Z8_TOBAC | 54 | – | – | + | + | – | – | – | – | |
In the table are shown the protein composition and the relative genes for each of the PSII types separated (Figure 2 inset). C2S2M2, C2S2, C2, C are the PSII-LHCII megacomplexes, PSII-LHCII supercomplexes, PSII dimers, and PSII monomers, respectively. *Unweighted spectrum count/MASS (kDa) = i; + = 0.25 ≤ i ≤ 0.50; ++ = 0.50 ≤ i ≤ 0.75; +++ = i > 0.75 (for details see Materials and Methods).