FIGURE 6.

Dark-induced Arabidopsis plants senescence of WT and transgenic lines (OX-1, OX-4, and OX-11). (A) Visual appearance of WT and transgenic plants after 4 days in dark. Scale bar = 2cm. (B) Trypan blue staining for cell death, NBT, and DAB staining for detecting levels of O₂^- and H₂O₂ production. Scale bar = 5mm. (C) The degree of leaves turning yellow. (D) Electrolyte leakage. (E) MDA content. Four-week-old Arabidopsis plants were used for inducing senescence, leaf numbers 3 or 4 were used for histochemical staining. Data are presented as mean ± standard error from three replicates with three biological repeats, and different letters above the columns indicate significant differences (LSD, P < 0.05) between wild type and transgenic lines.