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. 2015 Dec 10;6:1073. doi: 10.3389/fpls.2015.01073

FIGURE 8.

FIGURE 8

Effect of salt and drought stresses on seeding growth of WT and three independent transgenic lines (OX-1, OX-4, OX-11). (A) Photographs of WT and transgenic (OX-1, OX-4, and OX-11) plants watered normally and regularly (Control), watered with 400 mM NaCl for 18 days from 8 days old, deprived of water for 10 days of 18-days-old plants and re-watered for 24 h. (B) NBT and DAB staining of leaves from control plants watered normally and regularly. Scale bar = 5mm. (C) NBT and DAB staining of leaves under salt stress. Leaves were excised at 24 h after treated with 400 mM NaCl with 4-week-old plants. Scale bar = 5mm. (D) NBT and DAB staining of leaves under drought stress. Leaves were detached from plants deprived of water for 10 days with 18-days-old plants. Scale bar = 5mm. (E) Stress responsive related genes expression from leaves treated with 400 mM NaCl after 24 h. (F) Stress responsive related genes expression from leaves deprived of water for 10 days. Data are presented as mean ± standard error from three replicates with three biological repeats, and different letters above the columns indicate significant differences (LSD, P < 0.05) between wild type and transgenic lines.