Table 2.
Lymphocyte populations (%).
| Sham | Saline | A100 | A200 | A400 | |
|---|---|---|---|---|---|
| Blood | |||||
| T cells | 17.4 ± 0.8 | 13.1 ± 1.3∗ | 12.9 ± 0.8∗ | 13.0 ± 1.0∗ | 11.7 ± 0.8∗ |
| Th cells | 58.4 ± 1.0 | 51.4 ± 1.6∗ | 51.8 ± 1.5∗ | 53.4 ± 1.4 | 53.7 ± 1.4 |
| Tc cells | 32.9 ± 2.8 | 33.7 ± 3.3 | 36.0 ± 3.7 | 36.8 ± 2.8 | 35.1 ± 3.1 |
| B cells | 49.8 ± 1.6 | 31.1 ± 1.4∗ | 30.2 ± 2.6∗ | 25.6 ± 2.7∗ | 22.3 ± 3.0∗ |
| Spleen | |||||
| T cells | 24.2 ± 0.6 | 20.4 ± 1.0∗ | 19.7 ± 0.6∗ | 18.9 ± 0.7∗ | 19.6 ± 0.7∗ |
| Th cells | 47.6 ± 1.3 | 39.9 ± 1.2∗ | 42.5 ± 0.8∗ | 43.5 ± 1.0 | 44.1 ± 0.8 |
| Tc cells | 34.4 ± 0.7 | 36.1 ± 0.7 | 34.0 ± 0.6 | 33.3 ± 0.3 | 34.0 ± 1.1 |
| B cells | 47.1 ± 1.4 | 48.8 ± 1.4 | 48.9 ± 1.4 | 50.5 ± 1.4 | 49.1 ± 1.3 |
| Peyer's patches | |||||
| T cells | 8.4 ± 0.5 | 11.7 ± 1.0 | 15.6 ± 2.4∗ | 16.2 ± 1.8∗ | 17.4 ± 2.4∗ |
| Th cells | 53.8 ± 3.7 | 28.4 ± 1.3∗ | 27.3 ± 3.2∗ | 31.7 ± 4.1∗ | 29.1 ± 2.5∗ |
| Tc cells | 16.9 ± 2.0 | 14.4 ± 1.7 | 15.0 ± 2.8 | 16.6 ± 2.5 | 15.3 ± 2.5 |
| B cells | 77.8 ± 1.9 | 58.1 ± 2.4∗ | 52.7 ± 5.6∗ | 52.9 ± 5.2∗ | 53.6 ± 6.1∗ |
CD45-positive cells were gated to determine the population of lymphocytes by flow cytometry. Populations of T and B cells were determined as the percentages of CD3ε +CD19− and CD3ε −CD19+ cells among CD45-positive cells. Percentages of T helper (Th) and cytotoxic T (Tc) cells were, respectively, determined by CD4+CD8− and CD4−CD8+ cells in CD3ε-expressing T lymphocytes. Values are presented as the mean ± SEM. ∗Significantly different from the sham group (p < 0.05).
A100, A200, and A400, respectively, indicate treatment with 100, 200, and 400 mg Astragalus polysaccharides/kg of body weight.