Table 3.

T lymphocyte activation (%).

	Sham	Saline	A100	A200	A400
Spleen
CD69+ Th cells	10.4 ± 0.6	12.4 ± 1.2	14.5 ± 0.9∗	15.3 ± 0.8∗	16.6 ± 1.0∗
CD69+ Tc cells	4.6 ± 0.4	7.4 ± 1.4	7.0 ± 0.8	8.1 ± 1.5	8.3 ± 2.1
Peyer's patches
CD69+ Th cells	53.6 ± 2.5	38.3 ± 3.2∗	37.7 ± 2.5∗	45.6 ± 2.3	46.0 ± 4.0
CD69+ Tc cells	42.8 ± 6.2	45.3 ± 6.8	43.3 ± 6.3	42.5 ± 5.4	46.1 ± 5.7

T helper (Th) and cytotoxic T (Tc) cells were, respectively, identified by CD4⁺CD8⁻ and CD4⁻CD8⁺ cells among CD3ε-positive cells using a flow cytometer. Activated T cells were determined by the expression of CD69 on T-cell subsets. Values are presented as the mean ± SEM. ^∗Significantly different from the sham group (p < 0.05).

A100, A200, and A400, respectively, indicate treatment with 100, 200, and 400 mg Astragalus polysaccharides/kg of body weight.