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HuR, COX-2, and HO-1 mRNA expression in pancreatic cancer cells. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis revealed higher normalized HuR (A), COX-2 (B), and HO-1 (C) mRNA values in GEM treated cells compared to control cells (P < 0.05). Data from three independent experiments are shown. For normalization β-actin housekeeping gene and ΔΔCt method was used. HO-1: Heme oxygenase-1; COX-2: Cyclooxygenase-2; HuR: Human antigen R.
