Table 1.
Used primer and restriction enzyme at each polymorphism
| Polymorphisms | Forward primer | Reverse primer | Restriction enzyme1 |
| -238 G>A | 5’-AGA AGA CCC CCC TCG GAA CC2-3’ | 5’-ATC TGG AGG AAG CGG TAG TG-3' | MspI |
| -308 G>A | 5’-AGG CAA TAG GTT TTG AGG GCC AT-3' | 5’-TCC TCC CTG CTC CGA TTC CG-3' | NcoI |
| -857 C>T | 5′-AAG TCG AGT ATG GGG ACC CCC CGT TAA-3' | 5′-CCC CAG TGT GTG GCC ATA TCT TCT T-3' | AseI |
| -863 C>A | 5'-GGC TCT GAG GAA TGG GTT | 5'-CTA CAT GGC CCT GTC TTC GTT ACG-3' | MnlI |
| -1031 T>C | 5’-AGC AAG AGC TGT GGG GAG AA-3' | 5’-CCT GTA ACC CAT TCC TCA GAG CC-3' | BbsI |
1
All of the restriction enzymes were available from New England Biolabs (MA, United States) and we used the reaction conditions recommended by the instructions;
2
The underlined bases in the primer were mismatched with the wild-type sequence in order to introduce the restriction enzyme site.