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. 2015 Dec 10;6:1370. doi: 10.3389/fmicb.2015.01370

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Copyright © 2015 Huang, Song, Yang, Dong, Hu and Gao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of the His₆-DnaA (IV) in Escherichia coli cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the total Cyanothece 51142 cell proteins. Molecular mass markers (M) are indicated on the left and the positions of the protein are indicated on the right. Lane 1, supernatant of cell lysate; Lane 2, precipitant of cell lysate; Lane 3, breakthrough peak in the affinity chromatogram; Lane 4, targeted protein in the affinity chromatogram.