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. 2015 Dec 10;6:1370. doi: 10.3389/fmicb.2015.01370

FIGURE 4.

FIGURE 4

Interaction of the DnaA (IV) protein with the Cyanothece 51142 oriC. (A) Interaction of the DnaA (IV) protein with the Cyanothece 51142 oriC (5% gel). (1) Free oriC fragment of the circular chromosome. (2) Free oriC fragment of of the linear chromosome. (3) His6-DnaA (IV) binding to the circular chromosome oriC. (4) His6-DnaA (IV) binding to the linear chromosome oriC. (B) Interaction of the DnaA (IV) protein with the Cyanothece 51142 oriC (5% gel). (1) Free oriC fragment of the linear chromosome. (2–4) His6-DnaA (IV) binding to the linear chromosome oriC (10, 25, 50 nM). (5) Free oriC fragment of the circular chromosome. (6–8) His6-DnaA (IV) binding to the circular chromosome oriC (10, 25, 50 nM). (C) Interaction of the His6-DnaA (IV) protein with the oriC of the circular chromosome (5% gel). (1) oriC fragment of the circular chromosome [without DnaA (IV)]. (2) His6-DnaA (IV) protein (without the oriC fragment). (3–9) His6-DnaA (IV) binding to the circular chromosome oriC (3: 2.5; 4: 5.0; 5: 10.0; 6: 25.0; 7: 50.0; 8: 75.0; 9: 100.0 nM). (D) Interaction of the His6-DnaA (IV) protein with the oriC of linear chromosome (5% gel). (1) oriC fragment of the linear chromosome [without His6-DnaA (IV)]. (2) His6-DnaA (IV) protein (without the oriC fragment). (3–9) His6-DnaA (IV) binding to the linear chromosome oriC (3: 2.5; 4: 5.0; 5: 10.0; 6: 25.0; 7: 50.0; 8: 75.0; 9: 100.0 nM).