Table 1.
Bacterial strains and plasmids used in this study.
| Strain/plasmid | Genotype/relevant characteristics | Source |
|---|---|---|
| Escherichia coli DH5α | supE44, hsdR17, recA1, endA1, gyrA96, thi-1, relA1 | Novagen |
| E. coli BL21 (DE3) | Overexpression of recombinant protein His6-DnaA (IV) | Novagen |
| pET-28a(+) | Kanr, expression vector, His-tag coding sequence | Novagen |
| pEASY-T1 | Ampr, Kanr, T-vector for cloning PCR-amplified fragments | TransGen |
| pET28a-DnaA (IV) | Expression plasmid derived from pET-28a for the Domain IV of Cyanothece 51142 His6-DnaA (IV) | This study |
| pEASY-oriC-C | Cloning plasmid derived from pEASY-T1 of oriC region of circular chromosome | This study |
| pEASY-oriC-L | Cloning plasmid derived from pEASY-T1 of oriC region of circular chromosome | This study |