. 2015 Dec 10;6:1370. doi: 10.3389/fmicb.2015.01370

Table 2.

Oligonucleotides used in this study.

Names	Sequence(5′ to 3′)^a
dnaA-F	ATGACTATTTCCCCTCAATATATTTGGAACC
dnaA-R	TTAATTTTGATTCCGACTAGCAATATTAATG
dnaA(IV)-F	CGGGATCCATTTCCATTTCAGGACTATCGATGA
dnaA(IV)-R	CCAAGCTTTTAATTTTGATTCCGACTAGCAATA
oriC-C-F	GCTCTAACTTTCGCTTCTCTTGGAAGCCAT
oriC-C-R	ACGGCCCGACTGACTAAAGATAAATTGCT
oriC-L-F	CCGCCGTAGATATTTTAACGAAGGCCCCTC
oriC-L-R	TTGGTAAGCAACTTGTTGAGCCGTCAACTC
Cbox1F	GTCCTGTGGGGGTCTGTGGAAAACATTAAAATTC
Cbox1R	GAATTTTAATGTTTTCCACAGACCCCCACAGGAC
Cbox23F^b	CCTTTAAATTTTTCCACAGTTTTTCCACAGCATTA
Cbox23R^b	TAATGCTGTGGAAAAACTGTGGAAAAATTTAAAGG
Lbox23F^b	ATCTTGAATGTTGATAAATTTAAGTATTTATCCTCAACATTG
Lbox23R^b	CAATGTTGAGGATAAATACTTAAATTTATCAACATTCAAGAT
EboxF^b	GATCCTTGTTATCCACAGGGCAGTGC
EboxR^b	GCACTGCCCTGTGGATAACAAGGATC

^aRestriction sites are underlined; DnaA boxes are boxed.

^bPrior to use in the EMSA assay, the oligonucleotides were annealed with their complementary oligonucleotides. The boxed bases represent the DnaA box(es).

Ebox represents the E. coli DnaA box.