Table 2. Stability of reference gene expression under four experimental conditions.

Experimental conditions	Reference gene	geNorm		Normfider		BestKeeper		ΔCt
		Stability	Rank	Stability	Rank	Stability	Rank	Stability	Rank
Developmental stage	V-ATPase	0.909	3	0.399	1	0.548	3	1.277	1
	12S	1.060	8	0.879	9	0.837	9	1.400	3
	16S	1.010	6	0.756	7	0.822	8	1.362	2
	18S	1.035	7	0.760	8	0.892	10	1.437	8
	Actin	0.980	5	0.687	4	0.646	5	1.431	6
	EF1A	0.943	4	0.703	5	0.476	1	1.409	5
	28S	1.315	14	1.621	15	1.514	15	1.953	15
	GAPDH	1.143	11	1.069	11	1.188	14	1.556	11
	RSP24	0.720	1	0.723	6	0.606	4	1.476	9
	RPS18	0.720	1	0.666	3	0.522	2	1.434	7
	NADH	0.813	2	0.651	2	0.665	6	1.402	4
	HSP90	1.089	9	0.993	10	0.737	7	1.567	12
	HSP70	1.177	12	1.165	13	0.907	11	1.621	13
	RPL4	1.252	13	1.457	14	1.045	13	1.895	14
	Tubulin	1.117	10	1.071	12	0.935	12	1.540	10
	ArgK	1.694	15	4.263	16	3.690	16	4.348	16
Tissue	V-ATPase	0.816	13	0.917	14	1.114	14	1.457	14
	12S	0.515	3	0.153	1	0.491	11	1.007	3
	16S	0.600	6	0.518	7	0.426	6	1.077	5
	18S	0.540	4	0.321	2	0.185	1	1.000	1
	Actin	0.635	8	0.659	11	0.515	12	1.153	11
	EF1A	0.692	11	0.483	6	0.456	8	1.126	9
	28S	0.618	7	0.721	13	0.255	2	1.147	10
	GAPDH	0.443	1	0.572	8	0.361	4	1.086	6
	RSP24	0.656	9	0.705	12	0.458	9	1.169	12
	RPS18	0.674	10	0.465	5	0.438	7	1.110	8
	NADH	0.568	5	0.580	9	0.382	5	1.108	7
	HSP90	0.743	12	0.622	10	0.798	13	1.254	13
	HSP70	0.469	2	0.332	3	0.461	10	1.007	2
	RPL4	1.396	15	3.331	16	2.681	15	3.419	16
	Tubulin	0.443	1	0.453	4	0.377	3	1.049	4
	ArgK	1.107	14	3.049	15	2.687	16	3.154	15
Sex	V-ATPase	0.990	15	0.936	14	0.683	14	1.162	16
	12S	0.966	14	0.887	12	0.736	15	1.123	12
	16S	0.718	8	0.425	1	0.263	1	0.849	1
	18S	0.938	13	0.947	15	0.809	16	1.129	13
	Actin	0.867	11	0.963	16	0.647	12	1.151	15
	EF1A	0.903	12	0.880	11	0.612	11	1.113	11
	28S	0.824	10	0.921	13	0.656	13	1.130	14
	GAPDH	0.636	6	0.530	3	0.391	2	0.888	4
	RSP24	0.261	1	0.607	7	0.484	6	0.907	6
	RPS18	0.564	5	0.526	2	0.416	3	0.872	3
	NADH	0.688	7	0.603	6	0.437	4	0.912	7
	HSP90	0.485	4	0.651	8	0.534	8	0.926	9
	HSP70	0.261	1	0.579	4	0.503	7	0.871	2
	RPL4	0.761	9	0.724	10	0.452	5	1.006	10
	Tubulin	0.403	2	0.580	5	0.562	9	0.890	5
	ArgK	0.440	3	0.652	9	0.565	10	0.913	8
dsRNA	V-ATPase	0.786	15	0.900	16	0.784	16	1.050	16
	12S	0.293	1	0.294	3	0.279	4	0.634	3
	16S	0.362	2	0.257	2	0.203	2	0.628	2
	18S	0.293	1	0.147	1	0.187	1	0.586	1
	Actin	0.407	3	0.340	4	0.305	6	0.649	4
	EF1A	0.492	7	0.343	5	0.362	8	0.666	5
	28S	0.436	4	0.428	6	0.221	3	0.696	6
	GAPDH	0.559	9	0.569	9	0.449	9	0.794	9
	RSP24	0.527	8	0.477	7	0.480	11	0.729	8
	RPS18	0.595	10	0.681	12	0.461	10	0.868	12
	NADH	0.633	11	0.668	11	0.547	12	0.857	11
	HSP90	0.450	5	0.504	8	0.302	5	0.726	7
	HSP70	0.705	13	0.748	13	0.688	14	0.925	13
	RPL4	0.748	14	0.870	15	0.713	15	1.022	15
	Tubulin	0.673	12	0.786	14	0.680	13	0.944	14
	ArgK	0.469	6	0.616	10	0.344	7	0.801	10