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. 2015 Dec 10;5:18186. doi: 10.1038/srep18186

Table 5. Difference between Wild Type HaSCP-2 and Mutants in NBD-Cholesterol binding.

  WT Y51A Y51W F53A F53W F89A F110W I117M Q131A
Kda (μM) 2.78 2.65 1.00 3.07 3.41 3.27 3.24 1.80 3.77
P valueb − 0.023 0.09 0.031 0.026 0.038 0.019 0.043 0.034

aKd values were determined using the GraphPad Prism version 4.0 (single-binding site, nonlinear regression model).

bP values vs. wild type were given for Dunnett’s post T test between the wild type HaSCP-2 and each mutant after a repeated measures analysis of variance.