Table 1. Phenotype analysis of the wild type, ∆Fgmon1 and ∆Fgrab7 mutants, ∆Fgmon1/FgRAB7 ^Q67L and WT/FgRAB7 ^Q67L transformant in F. graminearum.

Strain	Colony Diameter (cm)a				Conidiation (x106/ml)b	Conidial Length (μm)c	Conidial Morphology (%)d		DONe (mg mg-1ergosterol)
	CM	V8	5xYEG	MM			≥3 Septa	≤2 Septa
PH-1	7.3 ± 0.1	5.8 ± 0.1	5.3 ± 0.1	5.1± 0.1	1.23 ± 0.16	47.77 ± 4.94	91.2 ± 2.2	8.8 ± 2.2	4.10 ±0.10
∆Fgmon1	2.5 ± 0.1*	2.6 ± 0.1*	2.5 ± 0.1*	1.6 ± 0.1*	0.21 ± 0.04*	32.90 ± 3.96*	45.8 ± 3.1*	54.2 ± 3.1*	0.016 ±0.001*
∆Fgrab7	1.3 ± 0.1*	2.1 ± 0.1*	1.5 ± 0.1*	0.8 ± 0.1*	0.18 ± 0.03*	28.82 ± 5.40*	47.3 ± 2.8*	52.7 ± 2.8*	0.01±0.001*
∆Fgmon1/FgMON1	7.2 ± 0.1	5.8 ± 0.1	5.4 ± 0.1	5.1 ± 0.1	1.18 ± 0.11	47.10 ± 5.82	89.0 ± 4.5	11.0 ± 4.5	NA
∆Fgrab7/FgRAB7	6.7 ± 0.1	5.6 ± 0.1	5.1 ± 0.1	4.7 ± 0.1	1.14 ± 0.17	47.36 ± 4.76	88.7 ± 2.7	11.3 ± 2.7	NA
∆Fgmon1/FgRAB7Q67L	NA	5.2 ± 0.1	NA	NA	1.20 ± 0.09	47.11 ± 5.02	89.2 ± 3.2	10.8 ± 3.2	NA
WT/FgRAB7Q67L	NA	5.7 ± 0.1	NA	NA	1.21 ± 0.18	46.75 ± 5.36	88.3 ± 2.5	11.7 ± 2.5	NA

^aColony diameter of the indicated strains on different media after 3 days incubation at 25 °C.

^bQuantification of the conidial production of the indicated strains form CMC cultures.

^cMeasurement of the conidial length of the indicated strains.

^dPercentage of the abnormal conidia of the indicated strains.

^ePercentage of DON production of each mutant in comparison with that of the wild-type. ±SD was calculated from three repeated experiments and asterisks indicate statistically significant differences (p < 0.01). NA, not assayed.