Table 2. Germination of the wild type, ∆Fgmon1 and ∆Fgrab7 mutants in YEPD medium.
Strain | Multi-polarity Germination (%) |
|||||||
---|---|---|---|---|---|---|---|---|
4 h | 6 h | 8 h | 10 h | 12 h | 14 h | 16 h | 18 h | |
PH-10 | 30.3 ± 2.5 | 48.3 ± 3.2 | 75.0 ± 3.0 | 89.3 ± 2.1 | 93.0 ± 2.0 | 95.0 ± 1.0 | 95.7 ± 0.5 | 97.0 ± 1.0 |
∆Fgmon1 | 11.0 ± 2.0* | 16.3 ± 1.5* | 23.7 ± 2.1* | 45.3 ± 1.5* | 65.3 ± 2.5* | 75.7 ± 3.0* | 86.3 ± 1.5* | 91.0 ± 1.0 |
∆Fgrab7 | 10.3 ± 1.5* | 20.7 ± 2.1* | 49.0 ± 2.6* | 83.0 ± 2.0 | 91.7 ± 1.5 | NA | NA | NA |
∆Fgmon1/FgMON1 | 29.7 ± 3.1 | 47.7 ± 2.5 | 73.0 ± 3.0 | 88.7 ± 2.5 | 92.0 ± 2.6 | 94.0 ± 1.0 | 94.7 ± 1.5 | 95.3 ± 1.5 |
∆Fgrab7/FgRAB7 | 30.0 ± 2.0 | 48.0 ± 2.0 | 72.7 ± 3.1 | 87.0 ± 1.7 | 93.7 ± 1.5 | NA | NA | NA |
±SD was calculated from three repeated experiments and asterisks indicate statistically significant differences (p < 0.01). NA, not assayed.