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. Author manuscript; available in PMC: 2016 Dec 3.
Published in final edited form as: Cell. 2015 Dec 3;163(6):1468–1483. doi: 10.1016/j.cell.2015.11.020

Figure 3. Orb2 monomer acts a translation repressor while amyloidogenic oligomer as an activator.

Figure 3

(A) Orb2 monomer represses translation and Orb2 oligomer increases translation in Δorb2 embryo extract. (B) Mutations of the M2 sequence that reduce Orb2 recruitment also attenuate the translational effect of monomeric and oligomeric Orb2. (C) Polysome profile analysis of in vitro translation reactions shows that the Orb2 monomer reduces, while the oligomer increases the relative abundance of translating mRNA in polysome fractions. (D) Primarily oligomeric Orb2 associates with FLAG-tagged 60S ribosomal subunits that are part of polyribosomes. Treatment with 30mM EDTA (pH8.0) or 20 unit/ml RNaseA that disrupts polysomes also reduces oligomeric Orb2 association. The extracts in the left are immunoprecipitated with anti-FLAG antibodies and probed for Orb2. Data is expressed as mean ± SEM and * <0.01, ** <0.001, *** <0.0001 indicates p value and ns indicates not significant. Please also see supplementary figure 3 and supplementary figure 4.