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. 2015 Dec 10;5:18029. doi: 10.1038/srep18029

Figure 2. Correlative cryo-fluorescence (A,B) and cryo-scanning electron microscopy (C,D) of Borrelia burgdorferi-GFP on the surface of human neuroblastoma cells grown on carbon-coated sapphire discs.

Figure 2

A series of images of one particular GFP-tagged spirochete (green) interacting with the cell counterstained with Hoechst 33342 (blue). Images of region of interest from low magnification FM to high magnification SEM. The cryo-SEM images were acquired after 10 minutes ice sublimation and deposition of Pt/Pd layer onto the sample surface. Scale bars: (A) 50 μm, (B) 25 μm, (C,D) 1 μm.