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. 2014 Dec 4;45(3):903–914. doi: 10.1002/eji.201445049

Figure 1.

Figure 1

Reduction of switched memory B-cell numbers. (A) Flow cytometric analysis of B-cell populations in the peripheral blood of healthy control (CTR, top row), and Down syndrome (DS, lower row) children. Plots showing gating selection of CD19+ B cells and identification of the transitional, mature-naive and memory populations are shown. Memory B cells express CD27. IgM (IgM+) and switched (IgM−) memory B cells were identified by the expression of IgM on gated CD27+ B cells. One representative example of the analysis performed on all 19 DS and 19 CTR children is shown. (B) Absolute numbers of transitional, mature-naive, memory, IgM, and switched memory B-cell populations were determined by flow cytometry. (C) Total serum IgM, IgG, and IgA levels in CTR and DS children were measured by ELISA. (B, C) Data are shown as median + IQR of 19 CTR and 19 DS donors. Statistical significance was calculated by the Mann–Whitney U-test.