Table 2.
Effects of mesenchymal stem cells derived from bone marrow and adipose tissue on myenteric neurons in TNBS-induced colitis
| Sham | TNBS | TNBS + BM-MSC | TNBS + AT-MSC | |
|---|---|---|---|---|
| Total number of myenteric neurons/2 mm2 | ||||
| 24 h | 263.8 ± 3.5 | 202.5 ± 8.4 †††† | 258.8 ± 2.9 **** | 242.8 ± 6.2 *** † |
| 72 h | 262.0 ± 5.1 | 204.0 ± 5.4 ††† | 258.8 ± 7.6 ** | 240.5 ± 10.0 ** † |
| Total number of nNOS-IR neurons/2 mm2 | ||||
| 24 h | 52.3 ± 1.8 | 70.0 ± 4.9 ††† | 55.0 ± 3.2 ** | 53.5 ± 2.6 ** |
| 72 h | 52.5 ± 1.0 | 70.5 ± 3.2 ††† | 55.3 ± 2.7 ** | 57.3 ± 1.7 * |
| Proportion of nNOS-IR neurons/2 mm2 (%) | ||||
| 24 h | 19.9 ± 0.9 | 34.8 ± 2.8 †††† | 21.3 ± 1.2 **** | 22.1 ± 1.1 **** |
| 72 h | 20.2 ± 0.3 | 34.6 ± 1.8 †††† | 21.4 ± 1.3 **** | 23.9 ± 0.7 *** |
| Total number of ChAT-IR neurons/2 mm2 | ||||
| 24 h | 163.5 ± 2.1 | 111.3 ± 5.7 †††† | 148.3 ± 4.6 **** | 144.5 ± 4.9 *** † |
| 72 h | 157.8 ± 6.3 | 112.3 ± 1.7 †††† | 147.5 ± 7.3 **** | 145.3 ± 3.4 *** |
| Proportion of ChAT-IR neurons/2 mm2 (%) | ||||
| 24 h | 62.0 ± 1.4 | 55.1 ± 2.9 | 57.3 ± 1.6 | 59.5 ± 0.9 |
| 72 h | 59.1 ± 0.9 | 55.2 ± 2.3 | 57.1 ± 3.0 | 60.7 ± 2.5 |
TNBS 2,4,6-trinitrobenzene sulfonic acid, BM-MSC bone marrow-derived MSC, AT-MSC adipose tissue-derived MSC, MSC mesenchymal stem cell, nNOS neuronal nitric oxide synthase, ChAT choline acetyltransferase, IR immunoreactive
*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, significantly different to TNBS; †p < 0.05, †††p < 0.001, ††††p < 0.0001, significantly different to sham