Table 1.
Results of molecular analyses of 596 ticks collected in 2007 in Hungary
| Haemaphysalis species | Tick stage or sex | Number of ticks per pool | PCR positive/all pools | Results of sequencing (length, % identity, sample number) | Location in Hungarya | Reference sequence | Accession number of sequence in this study (name of isolate) |
|---|---|---|---|---|---|---|---|
| H. inermis | male | 5 | 7/24 | Babesia crassa (410 bp, 98.6 %, 4×) | B, P, S | KF791205 | KT725848 (Bcr-Hu1) |
| female | 5 | 12/40 | Babesia crassa (410 bp, 98.6 %, 6×) | B, P, S | KF791205 | KT725848 (Bcr-Hu1) | |
| H. concinna | male | 3 | 10/29 | Babesia sp. Kh-Hc222 (409 bp, 99.8 %, 1×) | P | KJ486568 | KT725849 (BKh-Hu1) |
| Babesia sp. Irk-Hc133 (409 bp, 100 %, 2×) | B, P, A, C | KJ486563 | KT725852 (BIrk-Hu1) | ||||
| Theileria capreoli (439 bp, 99.8 %, 2×) | B, P | AY726008 | KT725850 (Tc-Hu1) | ||||
| Theileria sp. ZS TO4b (440 bp, 100 %, 1×) | P | DQ520836 | KT725851 (TZSTO4-Hu1) | ||||
| female | 3 | 5/16 | Babesia motasi (403 bp, 99.3 %, 1×) | B | AY260179 | KT725853 (Bmo-Hu1) | |
| Babesia sp. Irk-Hc133 (409 bp, 100 %, 1×) | B, P, A, C | KJ486563 | KT725852 (BIrk-Hu1) | ||||
| Theileria sp. ZS TO4 (440 bp, 100 %, 1×) | P | DQ520836 | KT725851 (TZSTO4-Hu1) | ||||
| nymph | 10 | 7/7 | Babesia sp. Irk-Hc133 (409 bp, 100 %, 1×) | B, P, A, C | KJ486563 | KT725852 (BIrk-Hu1) | |
| Theileria capreoli (439 bp, 99.8 %, 1×) | B, P | AY726008 | KT725850 (Tc-Hu1) | ||||
| Theileria sp. ZS TO4 (440 bp, 100 %, 1×) | P | DQ520836 | KT725851 (TZSTO4-Hu1) | ||||
| larva | 52 | 0/1 | - | - | - | ||
| H. punctata | male | 5 | 1/5 | Theileria sp. OT3 (160 bp, 100 %, 1×) | P | KF470868 | KT725854 (TOT3-Hu1) |
| female | 3 | 1/3 | Theileria sp. OT3 (160 bp, 100 %, 1×) | V | KF470868 | KT725854 (TOT3-Hu1) | |
| nymph | 1 | 0/1 | - | - | - |
a Abbreviations: B Börzsöny Mountains, P Pilis Mountains, S Szekszárd Hills, V Visegrád Mountains, A Ásotthalom, C Cserhát Mountains
bNote that Theileria sp. ZS TO4 and T. capreoli are 99.5 % identical, but for comparability with relevant literature data and because of their separate clustering on the phylogentic tree (Fig. 1) both names are maintained in the table and in the text