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. 2015 Dec 7;6:10099. doi: 10.1038/ncomms10099

Figure 4. β-adducin influences DARPP-32 cytonuclear localization in vivo.

Figure 4

(a) Dorsal striatum sections from WT or β-adducin KO mice were labelled with DARPP-32 antibody and nuclei were stained with DAPI. Scale bars, 10 μm. (b) The percentage of cells with DARPP-32 immunoreactivity intensity in the nucleus greater than or equal to the cytoplasm was quantified in the dorsal striatum (DS) or nucleus accumbens (NAc) of β-adducin KO mice and WT littermates in sections as in a. Independent data points are plotted and means±s.e.m. are shown, n=8–12 mice per group from 2 experiments; two-way ANOVA: region effect, F(1,36)=2.7, not significant (NS); genotype effect, F(1,36)=12.6, P<0.001; no interaction, F(1,36)=0.03; post hoc multiple comparison Šidák's test, β-adducin KO versus WT, *P<0.05. (c) Same as in b except that the fluorescence intensity ratio between nucleus and cytoplasm was quantified. Two-way ANOVA: region effect, F(1,36)=4.98, P=0.03; genotype effect, F(1,36)=44.7, P<10−4; no interaction, F(1,36)=0.052; Šidák's test, β-adducin KO versus WT, ***P<0.001, ****P<10−4. The nucleus (Nucl.) and perinuclear cytoplasm (Perinucl.) areas were measured in the DS (d) and NAc (e). No significant difference between genotypes was detected: two-way ANOVA: genotype effect, DS, F(1,36)=1.24, P=0.30; NAc, F(1,36)=0.16, P=0.69.