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A) Histology of PEC-Ag microbeads at day 21 and B) chondrogenic micromasses. Alcian Blue-PAS staining shows the presence of sulfated and neutral glycosaminoglycans (blue for sulfated, light purple for neutral, magenta for both). Arrowheads show GAG-positive masses in microbeads cultured in chondrogenic (CDT) medium. Large agglomerations of GAG were found in the 10:1 formulation. 50 μm scale bar (100 μm scale bar for chondrogenic micromass positive control).
