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Significantly elevated MMP9 in premetastatic lung with Gr-1+CD11b+ cells as the major resource. A, co-IF staining of Gr-1 (green) and MMP9 (red) in lungs at different day after tumor injection. Blue, nuclei. One experiment from three is shown. B, gelatin zymography of protein lysis from indicated organs of normal mice and mice bearing 14 day 4T1 tumors. One of three experiments is shown.
