Table 1.
qRT-PCR Primer sequences
| gene | Marker Type | Forward Primer | Reverse Primer |
|---|---|---|---|
| 18S | reference | AACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG |
| Adiponectin | adipogenic | GAATCATTATGACGGCAGCA | TCATGTACACCGTGATGTGGTA |
| PPARG | adipogenic | TCTTCCATCACGGAGAGGTC | GATGCACTGCCTATGAGCAC |
| Pref-1 | preadipocyte | TGTGCAGGAGCATTCGTACT | CGGGAAATTCTGCGAAATAG |
| Collagen IV | basement membrane | CACATTTTCCACAGCCAGAG | GTCTGGCTTCTGCTGCTCTT |
| Fibronectin-1 | basement membrane | ACTGGATGGGGTGGGAAT | GGAGTGGCACTGTCAACCTC |
| Lama2 | basement membrane | TGCATTCGAAGCAAGATTCA | CTCGAAGGCTCCCAGACT |
| Lama4 | basement membrane | AGGGTGCACATTCTCCTGAC | GAGACTAGCGACTCAGGCGT |
| cIDEA | beige | TGCTCTTCTGTATCGCCCAGT | GCCGTGTTAAGGAATCTGCTG |
| PGC1α | beige | TGAGAACCGCTAGCAAGTTT | TGTAGCGACCAATCGGAAAT |
| PRDM16 | beige | ACGCAGAACTTCTCGCTACC | ATGGGAGATGCTGACGGATA |
| UCP-1 | beige | ACTGCCACACCTCCAGTCATT | CTTTGCCTCACTCAGGATTGG |